首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人食欲素/阿立新B(OX-B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的OX-B含量。人食欲素/阿立新B（OX-B）是一种由下丘脑神经元分泌的神经肽，能促进食欲、调节睡眠和觉醒。研究发现，OX-B与食欲素受体结合后，可调节摄食行为、睡眠周期及情绪等生理过程。针对OX-B及其受体的研究，为治疗肥胖、失眠等疾病提供了新的治疗靶点。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，该产品适用于神经生物学研究中食欲素系统的功能解析、代谢调节机制探索，或睡眠障碍动物模型中的生物标志物分析，为中枢神经系统调控研究提供可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：OX-B
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays):CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human OX-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 107 | |||||
| Range % | 95-111 | ||||||
| 1:2 | Average % | 100 | |||||
| Range % | 93-104 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 86-97 | ||||||
| 1:8 | Average % | 100 | |||||
| Range % | 92-104 | ||||||
回收率：
| The recovery of human OX-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 91 | 84-95 | |||||
| EDTA plasma (n=4) | 108 | 97-112 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 40002.3122.3782.3452.212 20001.3861.3461.3661.233 10000.8200.8120.8160.683 5000.5230.5040.5140.381 2500.3700.3560.3630.230 1250.2780.2680.2730.140 62.50.2090.2030.2060.073 00.1320.1340.133 | |||||||
货期：3-5 working days
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