首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人μ型阿片受体(OPRM1)酶联免疫试剂盒，为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的OPRM1含量。OPRM1 是一个重要靶点。它在人体生理活动中发挥关键作用，与痛觉调控、成瘾机制等密切相关。研究上，主要聚焦于其介导信号转导的机制，通过明确其与配体结合模式等，为开发新型镇痛药、抗成瘾药物等提供理论支撑。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品适用于体外研究OPRM1表达水平与疼痛机制、成瘾行为调控或神经精神疾病的相关性，为镇痛药物开发、受体功能解析及信号通路研究提供可靠工具，尤其适合在细胞模型、动物实验或临床前研究中定量分析生物样本的OPRM1动态变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：OPRM1; MOR1; Mu-type opioid receptor; M-OR-1; MOR-1; Mu opiate receptor; Mu opioid receptor; MOP; hMOP
缩写：OPRM1
Uniprot No.：P35372
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human OPRM1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 85-101 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 88-103 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 83-105 | |||||
回收率：
| The recovery of human OPRM1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 85-99 | ||||
| EDTA plasma (n=4) | 98 | 95-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.1232.0912.1072.00310001.8691.8531.8611.7575001.5411.5281.5351.4312501.1121.0961.1041.0001250.7080.6950.7020.59862.50.5000.4870.4940.39031.250.2890.2790.2840.18000.1040.1030.104 | ||||||
货期：3-5 working days
×
