首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠网膜素(omentin)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的omentin含量。omentin是一个研究靶点。其背景上，它可能在人体生理过程中具有一定作用。研究机制方面，目前具体情况暂不明确，但推测它或参与脂肪代谢、炎症调节等生理活动，后续还需更多研究探索其在各生理及病理机制中的具体角色。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，科研人员可利用开展脂肪代谢机制研究、能量代谢相关疾病模型评估、药物干预效果分析等实验，为探索网膜素在能量稳态调控中的功能提供定量检测支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：omentin
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat omentin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:200 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:400 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:800 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of rat omentin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.4122.4682.4402.2815002.0682.0342.0511.8922501.5981.5671.5831.4241251.0351.0121.0240.86562.50.5680.5460.5570.39831.20.3540.3610.3580.19915.60.2470.2680.2580.09900.1580.1590.159 | ||||||
货期：3-5 working days
×
