| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TAOK1; TAOK2; TAOK3;;p-TAOK 1/2/3 (S181/S181/S177) |
| WB Predicted band size | Calculated MW: 116 kDa ; Observed MW: 110,130,140 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human TAOK 1/2/3 around the phosphorylation site of S181/S181/S177 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Phospho-TAOK1/2/3(S181+S181+S177)抗体的假设性参考文献示例(仅供参考，实际文献需通过数据库验证)：
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1. **文献名称**：*TAOK1 phosphorylation at Ser181 regulates autophagy via mTOR signaling*
**作者**：Zhang et al., 2018
**摘要**：研究揭示了TAOK1在Ser181位点的磷酸化通过调控mTOR通路影响自噬过程，使用特异性抗体验证了该修饰在营养胁迫下的动态变化。
2. **文献名称**：*Phosphorylation of TAOK2 Ser181 mediates stress fiber formation in cell migration*
**作者**：Lee et al., 2020
**摘要**：通过Phospho-TAOK2(S181)抗体发现，该位点的磷酸化激活TAOK2激酶活性，促进RhoA依赖的细胞骨架重组和迁移。
3. **文献名称**：*TAOK3 Ser177 phosphorylation modulates T-cell receptor signaling*
**作者**：Smith et al., 2019
**摘要**：利用Phospho-TAOK3(S177)抗体证明，T细胞激活中该位点的磷酸化通过MAPK通路增强IL-2分泌，提示其在免疫应答中的调控作用。
4. **文献名称**：*Development and validation of a pan-TAOK phospho-specific antibody*
**作者**：Chen et al., 2017
**摘要**：描述了针对TAOK1/2/3(S181/S181/S177)的多克隆抗体制备，验证其在Western blot和免疫荧光中的特异性，应用于多种细胞模型。
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**建议**：实际文献可通过以下方式检索：
- 在PubMed或Google Scholar搜索关键词：**TAOK1/2/3 phosphorylation S181/S177**
- 查阅抗体供应商(如CST、Abcam)的产品说明书引用文献。
- 结合信号通路(如MAPK、autophagy)筛选相关研究。
Phospho-TAOK1/2/3(S181+S181+S177) antibodies specifically detect the phosphorylated forms of TAO kinase isoforms 1. 2. and 3 at conserved serine residues (S181 in TAOK1/2 and S177 in TAOK3). These kinases belong to the STE20 family of serine/threonine kinases and play critical roles in regulating diverse cellular processes, including mitogen-activated protein kinase (MAPK) signaling, cytoskeletal reorganization, apoptosis, and cell cycle progression. Phosphorylation at these sites is associated with kinase activation, often triggered by cellular stress, DNA damage, or other stimuli that modulate TAOK activity.
TAOK1. TAOK2. and TAOK3 share structural homology but exhibit tissue-specific expression and functional divergence. TAOK1 is widely expressed and implicated in neuronal development, TAOK2 is enriched in testes and linked to cell polarity, while TAOK3 regulates immune responses and hematopoiesis. The phosphorylation status of these residues serves as a biomarker for their activation state, enabling researchers to study their involvement in signaling pathways.
These antibodies are essential tools for investigating TAOK-mediated signaling in disease models, such as cancer, neurodegenerative disorders, and immune dysregulation. Validation typically involves knockout controls or phosphatase treatment to confirm specificity. Cross-reactivity between isoforms should be considered due to sequence similarity. Applications include Western blotting, immunofluorescence, and immunoprecipitation to assess spatial-temporal activation patterns under physiological or pathological conditions.
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