首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人γ分泌酶酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的γ-Secretase含量。γ-Secretase是一种具有重要生物学意义的靶点。它参与多种生物过程，尤其在淀粉样前体蛋白（APP）的裂解过程中发挥关键作用。相关研究聚焦于其作用机制，旨在探索通过调控γ-Secretase来干预与APP裂解异常相关的疾病，如阿尔茨海默病。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品适用于神经科学领域的基础研究，例如阿尔茨海默病动物模型的组织样本分析、体外培养细胞中γ分泌酶活性调控实验，或药理学研究中抑制剂/激活剂对酶表达水平影响的动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：gamma-secretase
缩写：γ-Secretase
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human γ-Secretase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 91 | |
| Range % | 86-97 | ||
| 1:2 | Average % | 97 | |
| Range % | 91-103 | ||
| 1:4 | Average % | 95 | |
| Range % | 89-100 | ||
| 1:8 | Average % | 94 | |
| Range % | 89-98 |
回收率：
| The recovery of human γ-Secretase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 98-101 | ||||
| EDTA plasma (n=4) | 98 | 95-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.5032.4582.4812.382 10002.0752.0882.0821.983 5001.3141.3551.3351.236 2500.7410.7850.7630.664 1250.4010.3860.3940.295 62.50.3120.3210.3170.218 31.250.2030.2180.2110.112 00.0960.1010.099 | ||||||
货期：3-5 working days
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