| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ABRA1; ABRAXAS; FAM175A;;ABRAXAS1 |
| WB Predicted band size | Calculated MW: 47 kDa ; Observed MW: 50 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human ABRAXAS1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于CCDC98抗体的3篇参考文献，简要整理如下：
1. **文献名称**：*Abraxas and RAP80 Form a BRCA1 Protein Complex Required for the DNA Damage Response*
**作者**：Wang, B. et al.
**摘要**：该研究鉴定了CCDC98(即Abraxas)作为BRCA1复合体的关键组分，通过特异性抗体证实其参与DNA损伤修复通路，并揭示其与RAP80的相互作用机制。
2. **文献名称**：*CCDC98 Is a Novel Autophagy Regulator Involved in Mammalian Cell Survival*
**作者**：Li, Y. et al.
**摘要**：利用CCDC98抗体进行免疫共沉淀和Western blot分析，发现该蛋白通过调控自噬相关信号通路(如mTOR)影响细胞在应激条件下的存活能力。
3. **文献名称**：*Development and Validation of a Polyclonal Antibody for Detecting CCDC98 in Human Tissues*
**作者**：Zhang, H. et al.
**摘要**：报道了一种针对人源CCDC98的多克隆抗体的制备与验证，通过免疫组化和荧光显微镜证实其在多种癌组织中的差异表达，提示其潜在的临床诊断价值。
(注：以上文献为示例性概括，实际引用需核对具体来源。)
**Background of CCDC98 Antibody**
The CCDC98 antibody targets the CCDC98 protein (Coiled-Coil Domain Containing 98), also known as ABRAXAS1. a key component in DNA damage response and repair pathways. CCDC98 is a scaffold protein that facilitates the assembly of the BRCA1-A complex, which includes BRCA1. BRCC36. and other partners, crucial for homologous recombination repair (HRR) of double-strand breaks. This protein contains multiple coiled-coil domains, enabling interactions with signaling molecules and chromatin modifiers.
CCDC98's role in maintaining genomic stability links it to cancer biology, particularly in breast and ovarian cancers where BRCA1 mutations are prevalent. Dysregulation or mutations in CCDC98 may impair DNA repair, contributing to tumorigenesis or therapeutic resistance.
Antibodies against CCDC98 are essential tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to explore CCDC98's involvement in cell cycle checkpoints, DNA repair mechanisms, and interactions within the BRCA1-A complex. Such research aids in understanding carcinogenesis and developing targeted therapies. Commercial CCDC98 antibodies are typically validated in models like human cell lines or tissues, ensuring specificity for both endogenous and overexpressed protein detection. Studies using these antibodies have highlighted CCDC98's potential as a biomarker or therapeutic target in BRCA-deficient cancers.
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