Human bovine serum albumin (BSA) ELISA kit

货号: IPD-E08633h

Price： ￥2500

48T 96T

数量：

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产品描述：人的牛血清白蛋白(BSA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的BSA含量。BSA即牛血清白蛋白，背景上，它来源丰富、价格低廉，在生物医学领域广泛应用。研究机制方面，其可作为载体蛋白，结合药物、小分子等；也能模拟体内血浆环境，用于药物体外研究，还可用于酶活性测定、免疫分析等。试剂盒检测范围为0.312 μg/mL-80 μg/mL，灵敏度为0.312 μg/mL。本试剂盒通过预包被特异性抗体，利用样本中BSA与酶标记BSA竞争结合抗体的特性，结合显色反应实现定量分析，可广泛用于实验室评估样本制备过程中BSA的添加浓度、检测实验体系中的外源性BSA干扰，或作为生物制品生产过程中蛋白成分的质控工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：BSA
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：0.312 μg/mL-80 μg/mL
灵敏度：0.312 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human BSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-92
1:2	Average %	95
	Range %	91-105
1:4	Average %	95
	Range %	92-100
1:8	Average %	93
	Range %	85-98

回收率：

The recovery of human BSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-108
EDTA plasma (n=4)	97	90-110

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μg/mlOD1OD2Average　800.2860.2910.289　200.4350.4420.439　50.5890.5930.591　1.250.7990.8050.802　0.3121.2281.2371.233　01.7721.7821.777

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