首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人凝血酶酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的Thrombin含量。Thrombin即凝血酶，在凝血过程中起核心作用。其背景在于它是凝血瀑布反应的关键酶，可促使纤维蛋白原转化为纤维蛋白。研究机制聚焦于其促凝和抗凝双重作用，通过与受体结合调节细胞功能，还可作为靶点开发抗凝血药物。试剂盒检测范围为7.8 ng/mL-500 ng/mL，心血管疾病机制研究、抗凝药物体外效力评估、创伤修复模型分析等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：coagulation factor II (thrombin) ELISA Kit; Coagulation factor II ELISA Kit; F2 ELISA Kit; Factor II ELISA Kit; Prepro coagulation factor II ELISA Kit; Prothrombin ELISA Kit; prothrombin B-chain ELISA Kit; PT ELISA Kit; RPRGL2 ELISA Kit; serine protease ELISA Kit; THPH1 ELISA Kit; THRB ELISA Kit; THRB_HUMAN ELISA Kit; Thrombin heavy chain ELISA Kit
缩写：Thrombin
Uniprot No.：P00734
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：7.8 ng/mL-500 ng/mL
灵敏度：1.95 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Thrombin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 87 | ||||
| Range % | 80-95 | |||||
| 1:2000 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:4000 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:8000 | Average % | 95 | ||||
| Range % | 86-101 | |||||
回收率：
| The recovery of human Thrombin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-109 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected5002.7712.8062.7892.6562502.3472.3572.3522.2191251.6311.6951.6631.53062.51.0561.1041.0800.94731.20.5910.5760.5840.45115.60.3730.3890.3810.2487.80.2850.2960.2910.15800.1340.1320.133 | ||||||
货期：3-5 working days
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