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Human Retinoic Acid ELISA Kit

  • 中文名： 人视黄酸ELISA Kit
货号: IPD-E16712h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人视黄酸酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测样本中的Retinoic Acid含量。Retinoic Acid（视黄酸）是维生素A的衍生物，在胚胎发育、细胞分化等生理过程中起关键作用。研究机制上，它通过与核受体结合，调控基因表达，影响细胞生长、分化和凋亡。在肿瘤治疗等领域，视黄酸具有潜在应用前景。科研人员可借助开展视黄酸代谢调控研究、疾病模型中的分子机制探索，或评估药物干预对视黄酸信号通路的影响，为发育生物学、肿瘤微环境及免疫相关研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 缩写：
    Retinoic Acid
  • 种属：
    Homo sapiens (Human)
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Epigenetics and Nuclear Signaling
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human retinoic acid in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:100

    Average %

    93


    Range %

    86-100


    1:200

    Average %

    88


    Range %

    81-95


    1:400

    Average %

    96


    Range %

    89-102


    1:800

    Average %

    85


    Range %

    81-90


  • 回收率：

    The recovery of human retinoic acid spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    90

    83-97


    EDTA plasma (n=4)

    87

    82-94


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average




    100

    0.146

    0.138

    0.142




    50

    0.325

    0.337

    0.331




    25

    0.649

    0.618

    0.634




    12.5

    1.123

    1.059

    1.091




    6.25

    1.685

    1.601

    1.643




    0

    2.096

    2.103

    2.100





  • 货期：
    3-5 working days

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