首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胃蛋白酶原I酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PG I含量。PG I（胃蛋白酶原I）是胃中的一种消化酶，其生物学功能包括消化食物中的蛋白质。研究显示，PG I在心血管系统中具有重要作用，其合成能力由内向外依次减弱，以内皮细胞合成能力最强。PG I的研究机制涉及其对心血管系统的影响，以及其在胃癌诊断中的应用。试剂盒检测范围为6.25 ng/mL-400 ng/mL，支持科研人员高效开展胃功能研究、胃黏膜损伤机制探索或药物干预效果评估等实验。其宽泛的线性范围和多类型样本兼容性，特别适用于纵向研究或不同实验条件间的数据比对，为胃相关疾病的分子机制解析及生物标志物筛选提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：PG I
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：1.56 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human pepsinogen I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 106 | ||||
| Range % | 100-115 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 94-112 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human pepsinogen I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 97 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected4002.3962.2522.3242.2072001.6121.7361.6741.5571001.1041.0541.0790.962500.6620.6390.6510.534250.3990.4240.4120.29512.50.2920.3090.3010.1846.250.2050.1990.2020.08500.1180.1150.117 | ||||||
货期：3-5 working days
×
