| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ENPP5;;ENPP5 |
| WB Predicted band size | 55 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human ENPP5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于ENPP5抗体的3篇参考文献示例(注：以下内容为虚构，仅用于示例用途)：
1. **文献名称**：*Characterization of a Novel Monoclonal Antibody Against ENPP5 for Sperm Function Studies*
**作者**：Smith J. et al. (2018)
**摘要**：本研究开发了一种针对ENPP5的新型单克隆抗体，并通过免疫印迹和免疫荧光验证其特异性。抗体成功用于检测小鼠精子中ENPP5的表达，并发现其在精子获能过程中可能参与调控细胞外核苷酸代谢。
2. **文献名称**：*ENPP5 Expression in Ovarian Cancer and Its Detection Using a Polyclonal Antibody*
**作者**：Zhang L. et al. (2020)
**摘要**：通过制备兔源多克隆抗体，作者分析了ENPP5在卵巢癌组织中的表达水平。结果显示，ENPP5在肿瘤微环境中高表达，且与患者预后不良相关，抗体在免疫组化中表现出高灵敏度和特异性。
3. **文献名称**：*Role of ENPP5 in Zebrafish Development: Insights from Antibody-Based Knockdown Experiments*
**作者**：Garcia R. et al. (2019)
**摘要**：利用ENPP5特异性抗体进行功能阻断实验，发现ENPP5在斑马鱼胚胎早期发育中调控嘌呤信号通路。抗体通过显微注射干扰蛋白活性，导致胚胎发育缺陷，提示其关键作用。
(注：实际研究中请通过PubMed或Google Scholar检索真实文献。)
ENPP5 (Ectonucleotide Pyrophosphatase/Phosphodiesterase 5) is a member of the ENPP family of ectoenzymes, which hydrolyze extracellular nucleotides to regulate purinergic signaling. It is a type II transmembrane protein with a conserved catalytic domain, primarily expressed in specific tissues such as the brain, testis, and placenta. ENPP5 is implicated in modulating nucleotide-dependent processes, including cell adhesion, differentiation, and inflammation, by controlling extracellular levels of ATP, ADP, and other nucleotides. Its precise physiological roles remain less characterized compared to other ENPPs (e.g., ENPP1-3), though studies suggest involvement in spermatogenesis, neural development, and immune regulation.
Antibodies targeting ENPP5 are essential tools for studying its expression, localization, and function. These antibodies are typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. They enable techniques such as Western blotting, immunohistochemistry, and flow cytometry to detect ENPP5 in cellular or tissue samples. Validation often includes confirming specificity via knockout controls or siRNA silencing. Research applications focus on elucidating ENPP5’s role in diseases, including cancer (e.g., glioma, prostate cancer) and neurological disorders, where altered nucleotide signaling may contribute to pathogenesis. Commercial ENPP5 antibodies vary in clonality, epitope recognition, and conjugate labels, requiring careful selection based on experimental needs. Ongoing studies aim to clarify ENPP5's therapeutic potential as a biomarker or drug target.
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