首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人黑色素酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清样本中的melanin含量。Melanin即黑色素，是一种广泛存在于人和动物体内的生物色素。其在皮肤颜色形成、抵御紫外线等方面发挥重要作用。研究机制上，主要聚焦于其合成途径、调控因素等，旨在了解相关机制以开发美白、治疗色素异常疾病等方面的方法。试剂盒检测范围为12.5 pg/mL-1000 pg/mL，适用于皮肤生理机制、色素代谢调控及黑色素相关疾病模型的科学研究；适用于体外培养模型、动物实验样本或人体血清中黑色素水平的动态监测；可支持黑色素生成调控机制、光老化研究、美白成分筛选等基础科研需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：melanin
种属：Homo sapiens (Human)
样本类型：serum
检测范围：12.5 pg/mL-1000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human melanin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 89-102 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-100 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 92-97 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 90-101 | |||||
回收率：
| The recovery of human melanin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-105 | ||||
| EDTA plasma (n=4) | 96 | 91-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 10000.1450.1470.146 3750.2760.2860.281 187.50.4020.4130.408 62.50.5530.5620.558 12.51.0511.0841.068 02.4532.5672.510 | ||||||
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