| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MLC 2A; MLC20; MRLC2; MYL12B; MYLC2B; ;Myosin light chain 12B |
| WB Predicted band size | Calculated MW: 20 kDa ; Observed MW: 18 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Myosin light chain 12B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是3篇关于MYL12B抗体的参考文献摘要(基于公开研究数据模拟，非真实文献)：
1. **"MYL12B as a regulator of cytoskeletal dynamics in cancer cell migration"**
- 作者：Chen et al. (2018)
- 摘要：研究通过MYL12B抗体检测其在乳腺癌细胞中的表达，发现其通过调控肌球蛋白轻链磷酸化影响细胞迁移，提示其在肿瘤转移中的作用。
2. **"Development of a novel monoclonal antibody for MYL12B and its application in hepatocellular carcinoma diagnosis"**
- 作者：Wang et al. (2020)
- 摘要：报道一种高特异性MYL12B单克隆抗体的开发，并通过免疫组化验证其在肝癌组织中的高表达，提出其作为潜在诊断标志物的价值。
3. **"MYL12B interacts with ARHGEF2 to mediate cytokinesis in HeLa cells"**
- 作者：Smith et al. (2016)
- 摘要：利用MYL12B抗体进行共免疫沉淀实验，揭示其与ARHGEF2的相互作用机制，阐明其在细胞分裂末期胞质分裂中的关键功能。
4. **"Proteomic profiling identifies MYL12B as a biomarker for drug resistance in colorectal cancer"**
- 作者：Li et al. (2022)
- 摘要：通过Western blot和免疫荧光技术结合MYL12B抗体，发现其在化疗耐药结直肠癌患者中显著上调，提示其与耐药性相关。
建议通过PubMed或Google Scholar以“MYL12B antibody”为关键词检索获取真实文献。
The MYL12B antibody targets the protein encoded by the MYL12B gene, which belongs to the myosin light chain (MLC) family. MYL12B, also known as myosin regulatory light chain 12B (MLC-2B), is a 20 kDa protein that regulates the activity of non-muscle myosin II (NMII), a motor protein critical for cytoskeletal dynamics. It binds to the heavy chain of NMII, enabling ATPase activity and facilitating actomyosin contractility, which drives processes like cell migration, cytokinesis, and maintenance of cell shape. Phosphorylation of MYL12B at specific residues (e.g., Ser19/Thr18) modulates its interaction with NMII, influencing cellular tension and motility.
MYL12B is ubiquitously expressed but plays distinct roles in various tissues, particularly in smooth muscle and non-muscle cells. Dysregulation of MYL12B has been implicated in pathological conditions, including cancer metastasis, cardiovascular diseases, and neurological disorders. Antibodies against MYL12B are widely used in research to study cytoskeletal organization, cell polarity, and mechanotransduction signaling (e.g., Rho/ROCK pathways). They are essential tools in techniques like Western blotting, immunofluorescence, and immunohistochemistry to localize and quantify MYL12B expression. These antibodies also aid in exploring MYL12B's role as a potential biomarker or therapeutic target in diseases linked to aberrant cell motility or contractility.
×
