Rabbit Monoclonal MRPS15 Antibody

The MRPS15 antibody is a tool used to detect mitochondrial ribosomal protein S15 (MRPS15), a key component of the small subunit (28S) of the mitochondrial ribosome. Encoded by the nuclear gene MRPS15. this protein plays a critical role in mitochondrial protein synthesis, particularly for subunits of the oxidative phosphorylation (OXPHOS) complexes essential for ATP production. Mitochondrial ribosomes differ structurally and functionally from cytosolic ribosomes, reflecting their specialized role in translating mitochondrion-encoded mRNAs. MRPS15 antibodies are widely employed in research to study mitochondrial dysfunction, which is linked to metabolic disorders, neurodegenerative diseases, and cancer. They are used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess protein expression levels, subcellular localization, and mitochondrial ribosome assembly. Studies have implicated MRPS15 dysregulation in conditions such as colorectal cancer and Leigh syndrome, highlighting its potential as a biomarker or therapeutic target. Commercial MRPS15 antibodies are typically raised against specific epitopes, with validation including knockout cell line controls to confirm specificity. Researchers must consider cross-reactivity with homologous proteins and tissue-specific expression patterns when interpreting results. Proper validation ensures reliable detection, aiding investigations into mitochondrial biology and disease mechanisms.