Human L-Carnitine ELISA Kit

大包装询价

货号: IPD-E13606h

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人左旋肉碱/L-肉碱酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织匀浆样本中的L-Carnitine含量。L-Carnitine即左旋肉碱，它是一种促使脂肪转化为能量的类氨基酸，存在于人体中。在研究机制方面，它能将长链脂肪酸转运到线粒体中进行氧化，从而促进脂肪代谢。临床上还可用于辅助治疗一些疾病，在减肥等领域也备受关注。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于代谢调控机制研究、运动医学评估、营养代谢实验及药物开发中的生物标志物检测；满足实验室对脂肪酸代谢、心肌能量代谢及线粒体功能相关研究的精准定量需求；为探索L - 肉碱在能量代谢异常、肥胖症或运动恢复等领域的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：
L-Carnitine
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, urine, tissue homogenates
检测范围：
0.312 ng/mL-20 ng/mL
灵敏度：
0.078 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Biochemicals
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human L-Carnitine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	88
	Range %	80-96
1:400	Average %	97
	Range %	91-105
1:800	Average %	99
	Range %	92-110
1:1600	Average %	100
	Range %	90-110

回收率：

The recovery of human L-Carnitine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	82-110
EDTA plasma (n=4)	97	90-110

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














ng/ml	OD1	OD2	Average	Corrected
20	2.749	2.639	2.694	2.543
10	2.010	1.976	1.993	1.842
5	1.303	1.294	1.299	1.148
2.5	0.889	0.839	0.864	0.713
1.25	0.561	0.524	0.543	0.392
0.625	0.371	0.359	0.365	0.214
0.312	0.256	0.239	0.248	0.097
0	0.155	0.147	0.151

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.