| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Npl3; RBP56; RNA binding protein 56; TAF; TAF15; TAF2N; TAFII68;;TAF15 |
| WB Predicted band size | Calculated MW: 62 kDa ; Observed MW: 75 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human TAF15 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇涉及TAF15抗体的参考文献及其简要摘要：
1. **文献名称**: *TAF15 is implicated in the pathological aggregation of FTLD-TDP through its prion-like domain*
**作者**: Neumann M, et al.
**摘要**: 本研究通过免疫组织化学和Western blot技术，发现TAF15蛋白在额颔叶变性(FTLD-TDP)患者的神经元中异常聚集，其朊病毒样结构域可能通过液-液相分离驱动病理蛋白聚集，提示TAF15抗体在神经退行性疾病诊断中的潜在价值。
2. **文献名称**: *Fusion of the TAF15 gene with FUS in a radiation-induced bone sarcoma*
**作者**: Andersson MK, et al.
**摘要**: 利用TAF15特异性抗体检测肉瘤样本，首次报道了辐射诱导骨肉瘤中TAF15-FUS融合基因的存在，揭示了TAF15的异常重排可能通过干扰RNA代谢促进肿瘤发生，为癌症分子机制研究提供了新方向。
3. **文献名称**: *Phosphorylation regulates TAF15 subcellular localization and DNA damage response*
**作者**: Jobert L, et al.
**摘要**: 研究通过免疫荧光和共聚焦显微术，证明DNA损伤条件下TAF15的磷酸化修饰调控其核质转位，并影响PARP1介导的修复通路，提示TAF15抗体在细胞应激反应研究中的工具价值。
4. **文献名称**: *TAF15 controls a pro-inflammatory RNA splicing switch in neurodegeneration*
**作者**: Kapeli K, et al.
**摘要**: 利用TAF15抗体进行RNA免疫沉淀测序(RIP-seq)，发现TAF15通过结合促炎因子的pre-mRNA调控异常剪接，在肌萎缩侧索硬化症(ALS)中加剧神经炎症，为靶向TAF15的治疗策略提供依据。
这些研究均依赖TAF15抗体开展蛋白定位、互作或功能分析，覆盖神经疾病、癌症及基础分子机制领域。
TAF15 (TATA-box binding protein-associated factor 15) is a member of the FET protein family, which also includes FUS and EWSR1. These proteins are characterized by conserved N-terminal domains rich in glutamine, serine, and tyrosine residues (Q/S/Y-rich domain), along with RNA recognition motifs (RRM) and C-terminal prion-like domains. TAF15 functions as a multifunctional RNA/DNA-binding protein involved in transcription initiation, RNA splicing, and DNA damage response. It interacts with RNA polymerase II and other core transcription machinery components, playing roles in regulating gene expression.
Antibodies targeting TAF15 are critical tools for studying its biological functions and disease associations. TAF15 is implicated in several pathologies, including neurodegenerative diseases like amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), where cytoplasmic mislocalization and aggregation of FET proteins are observed. Additionally, chromosomal translocations involving TAF15 are linked to certain sarcomas and leukemias, producing oncogenic fusion proteins. Researchers use TAF15 antibodies in techniques such as Western blotting, immunohistochemistry, and immunoprecipitation to investigate its expression, localization, and interactions. Specific antibodies may distinguish between wild-type TAF15 and disease-associated variants or post-translationally modified forms. Validating antibody specificity is crucial, as FET proteins share structural homology, potentially leading to cross-reactivity. Studies employing TAF15 antibodies contribute to understanding its role in phase-separated condensates, RNA metabolism, and disease mechanisms, offering insights for therapeutic targeting.
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