首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人F-肌动蛋白酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的F-actin含量。F-actin即丝状肌动蛋白，是细胞骨架的重要组成部分。其在细胞形态维持、运动迁移、物质运输等过程中发挥关键作用。研究机制上，主要聚焦于其聚合和解聚动态平衡调控，以及与多种结合蛋白相互作用，影响细胞生理和病理状态。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于细胞骨架动态研究、细胞力学特性分析、心血管疾病或肿瘤转移机制探索等科研领域，尤其可为细胞应激反应、病理模型构建及药物干预效果评估提供可靠的分子检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：F-actin
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human F-actin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human F-actin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-101 | ||||
| EDTA plasma (n=4) | 88 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.6912.6072.6492.430202.3042.1392.2222.003101.8041.8621.8331.61451.3191.3311.3251.1062.50.8540.9070.8810.6621.250.5920.5490.5710.3520.6250.3850.3670.3760.15700.2260.2120.219 | ||||||
货期：3-5 working days
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