首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人锁链素酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液、组织匀浆样本中的DES含量。人锁链素是一种存在于人体结缔组织中的弹性蛋白交联氨基酸，负责弹性蛋白的弹性和黄色特质。其研究涉及弹性蛋白的降解与合成，以及与皮肤衰老和疾病的关系。试剂盒检测范围为0.156 ng/mL-10 ng/mL，可满足科研领域中对细胞培养模型、动物实验或临床前样本的锁链素水平分析需求，为探索肠道吸收障碍、炎症性疾病的分子机制或药物干预效果评估提供精准的实验工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：DES
种属：Homo sapiens (Human)
样本类型：serum, urine, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 87-99 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 82-92 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 94-106 | |||||
回收率：
| The recovery of human DES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.9322.9022.9172.82752.1732.0982.1362.0462.51.4661.4211.4441.3541.250.7880.7730.7810.6910.6250.4620.4450.4540.3640.3120.2980.2790.2890.1990.1560.2060.1910.1990.10900.0930.0870.090 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human DES antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled DES antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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