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产品描述：人抗菌肽LL-37酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LL-37含量。LL-37是一种存在于人体中的抗菌肽，具有广谱抗菌和抗肿瘤活性。其研究机制涉及抗菌、免疫调节、抗内毒素、趋化、刺激新生血管生成和诱导细胞凋亡等多方面。LL-37在多种胃肠道疾病中表达量变化，提示其在消化系疾病进展中发挥作用。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于基础科研中探索LL-37在感染模型、免疫调控机制或皮肤相关疾病中的动态变化，例如通过分析不同实验条件下（如病原体刺激、药物干预）生物样本中LL-37的表达差异，为免疫学研究或治疗策略开发提供数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：LL-37
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LL-37 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 85 | ||||
| Range % | 80-92 | |||||
| 1:10 | Average % | 98 | ||||
| Range % | 91-105 | |||||
| 1:20 | Average % | 98 | ||||
| Range % | 92-110 | |||||
| 1:40 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human LL-37 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 104 | 92-112 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.4202.4682.4442.24651.8751.8951.8851.6872.51.3581.3781.3681.1701.250.9410.9560.9490.7510.6250.5520.5890.5710.3730.3120.4310.4530.4420.2440.1560.3780.3890.3840.18600.1920.2040.198 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human antibacterial peptide LL-37 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled antibacterial peptide LL-37 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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