| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CaMKN; POPX1; PP2CH; Ppm1e;;Protein phosphatase 1E |
| WB Predicted band size | Calculated MW: 84 kDa ; Observed MW: 90 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Protein phosphatase 1E |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于PPM1E抗体的相关文献摘要(信息基于公开研究数据模拟，实际文献请通过学术数据库核实)：
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1. **文献名称**: "PPM1E regulates AMP-activated protein kinase signaling through direct dephosphorylation"
**作者**: Suzuki A et al.
**摘要**: 研究揭示了PPM1E通过去磷酸化AMPKα亚基调控能量代谢通路，抗体用于检测PPM1E在肝脏组织中的表达变化及其与糖尿病模型中的功能关联。
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2. **文献名称**: "The role of PPM1E in neuronal oxidative stress response"
**作者**: Chen L et al.
**摘要**: 利用PPM1E特异性抗体，研究发现该磷酸酶通过调控ATM/p53通路减轻神经元氧化损伤，为神经退行性疾病治疗提供潜在靶点。
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3. **文献名称**: "Development and validation of a monoclonal antibody for PPM1E in cancer cell lines"
**作者**: Kim H et al.
**摘要**: 报道了一种高特异性抗PPM1E单克隆抗体的开发，验证其在乳腺癌细胞中检测PPM1E蛋白水平的能力，并发现其低表达与化疗耐药性相关。
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4. **文献名称**: "PPM1E modulates cardiac hypertrophy via calcineurin/NFAT signaling"
**作者**: Wang Y et al.
**摘要**: 通过免疫沉淀(使用PPM1E抗体)证明PPM1E抑制病理性心肌肥厚，机制涉及对钙调磷酸酶的去磷酸化作用及下游NFAT信号抑制。
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*注：以上内容为模拟生成，实际文献需通过PubMed、Google Scholar等平台检索确认。建议结合关键词“PPM1E antibody”或“PPM1E phosphatase”查找最新研究。*
The PPM1E antibody is a crucial tool for studying the protein phosphatase manganese-dependent 1E (PPM1E), a serine/threonine phosphatase belonging to the PP2C family. PPM1E, also known as POPX2. plays a regulatory role in diverse cellular processes, including stress response, cell cycle progression, and metabolic signaling, by dephosphorylating key substrates. It is implicated in pathways such as AMPK/mTOR, p53. and Ca²⁺/calmodulin-dependent kinase (CaMK), making it a focus in cancer, cardiovascular diseases, and neurodegenerative disorders. For example, PPM1E modulates AMPK activity to influence energy homeostasis and may act as a tumor suppressor or promoter depending on cellular context. In Alzheimer's research, it interacts with tau phosphorylation mechanisms.
The PPM1E antibody enables detection and quantification of PPM1E expression in experimental models, facilitating studies on its localization, expression levels, and interaction partners. It is widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Validation of antibody specificity often involves knockout controls or siRNA-mediated PPM1E knockdown. Commercial PPM1E antibodies are typically raised against conserved epitopes, with rabbit or mouse monoclonal variants available. Researchers utilize these tools to explore PPM1E's dual roles in disease progression and metabolic regulation, particularly its context-dependent oncogenic or tumor-suppressive behaviors. Its study contributes to therapeutic targeting strategies for conditions linked to phosphatase dysregulation.
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