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产品描述：人blca-4酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织匀浆样本中的BLCA-4含量。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于科研领域中对膀胱癌发病机制、肿瘤微环境调控及生物标志物筛查的研究，支持多种生物样本类型的平行检测，为体外实验提供稳定可靠的定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：BLCA-4
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human blca-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 84-91 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 96-103 | |||||
| 1:4 | Average % | 104 | ||||
| Range % | 101-107 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-99 | |||||
回收率：
| The recovery of human blca-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 84-90 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 402.5952.5402.5682.452 202.2082.3652.2872.171 101.8231.8041.8141.698 51.1951.1751.1851.069 2.50.6980.6780.6880.572 1.250.4180.4320.4250.309 0.6250.2710.2760.2740.158 00.1150.1170.116 | ||||||
货期：3-5 working days
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