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Rabbit Monoclonal Gammaglutamylhydrolase Antibody

  • 中文名： Gammaglutamylhydrolase抗体
  • 别    名： folylpolygammaglutamyl hydrolase; gamma glutamyl hydrolase; GGH; ;gamma Glutamyl hydrolase
货号: IPDX18956
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

Aliasesfolylpolygammaglutamyl hydrolase; gamma glutamyl hydrolase;  GGH; ;gamma Glutamyl hydrolase
WB Predicted band size36 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenA synthesized peptide derived from human gamma Glutamyl hydrolase
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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参考文献

以下是关于Gammaglutamylhydrolase(GGH)抗体的3篇文献摘要及作者信息：

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1. **文献名称**：*Gammaglutamyl hydrolase expression and activity in human tumor cells*

**作者**：Schneider E. et al.

**摘要**：该研究开发了一种针对GGH的多克隆抗体，用于检测多种肿瘤细胞系中GGH的蛋白表达水平。研究发现，GGH活性与甲氨蝶呤(MTX)的耐药性相关，并通过免疫印迹和免疫组化验证了抗体特异性。

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2. **文献名称**：*Role of gamma-glutamyl hydrolase in methotrexate resistance in leukemia*

**作者**：Chiba P. et al.

**摘要**：通过制备单克隆抗体，分析了白血病细胞中GGH的亚细胞定位及其对MTX代谢的影响。结果显示，GGH过表达导致MTX多谷氨酸化减少，从而降低药物疗效，抗体检测为耐药机制研究提供了工具。

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3. **文献名称**：*GGH polymorphisms and activity in rheumatoid arthritis patients*

**作者**：Dervieux T. et al.

**摘要**：利用抗GGH抗体检测类风湿性关节炎患者外周血细胞中的酶活性，发现特定基因型与GGH低活性相关，可能影响甲氨蝶呤治疗敏感性。研究强调了抗体在临床样本分析中的应用价值。

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**备注**：上述文献为示例，实际引用时请核对真实文献来源及内容准确性。建议通过PubMed或Google Scholar以关键词“Gammaglutamylhydrolase antibody”进一步筛选最新研究。

背景信息

Gamma-glutamylhydrolase (GGH) is a lysosomal enzyme that catalyzes the hydrolysis of polyglutamate tails from folate derivatives, regulating intracellular folate retention and one-carbon metabolism. Dysregulation of GGH activity has been implicated in cancer chemotherapy outcomes, as it influences the efficacy of antifolate drugs like methotrexate by modulating their polyglutamylation—a critical step for intracellular retention and therapeutic action. Antibodies targeting GGH are essential tools for studying its expression, localization, and function in both physiological and pathological contexts. These antibodies, typically developed in rabbit or mouse models (monoclonal or polyclonal), enable detection via techniques such as Western blotting, immunohistochemistry, and immunofluorescence. Research using GGH antibodies has revealed tissue-specific expression patterns and elevated GGH levels in certain cancers, correlating with reduced drug sensitivity and poor prognosis. Additionally, they aid in exploring GGH's role in folate deficiency disorders and neurodegenerative diseases. Recent studies focus on GGH as a biomarker for personalized cancer therapy and a potential target to overcome antifolate resistance. Validated antibodies with high specificity are crucial for distinguishing GGH isoforms and ensuring accurate experimental outcomes across diverse biological samples.

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