首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人3-硝基酪氨酸酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的3-NT含量。3-NT（神经营养素-3）是一种由人类NTF3基因编码的蛋白质，属于神经生长因子（NGF）家族。它对中枢神经系统和周围神经系统的特定神经元有调控活性，支持神经细胞的生存和分化，并促进新神经元的生长和分化。3-NT通过与其受体TrkC和TrkB结合，激活细胞信号传导，影响神经发生和神经元功能。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外研究领域，如氧化应激相关疾病模型的分子机制探索、抗氧化药物筛选及药效评估等实验场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：3-NT
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human 3-NT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 82-98 | |||||
| 1:4 | Average % | 104 | ||||
| Range % | 99-111 | |||||
| 1:8 | Average % | 86 | ||||
| Range % | 82-96 | |||||
回收率：
| The recovery of human 3-NT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-97 | ||||
| EDTA plasma (n=4) | 91 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.6552.7522.7042.55352.1112.2752.1932.0422.51.4861.4651.4761.3251.250.7690.7990.7840.6330.6250.4850.4620.4740.3230.3120.3720.3990.3860.2350.1560.2510.2640.2580.10700.1520.1500.151 | ||||||
货期：3-5 working days
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