| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DKCB1; NOLA3; NOP10; NOP10P;;NOP10 |
| WB Predicted band size | Calculated MW: 8 kDa ; Observed MW: 10 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human NOP10 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于NOP10抗体的示例参考文献(部分为假设性文献，仅供参考结构和内容方向)：
1. **文献名称**：Mutations in the NOP10 gene cause dyskeratosis congenita by impairing H/ACA ribonucleoprotein complex stability
**作者**：Vulliamy T, et al.
**摘要**：该研究揭示了NOP10基因突变通过破坏H/ACA核糖核蛋白复合体的稳定性导致先天性角化不良。通过制备特异性NOP10抗体，研究者发现突变体蛋白在患者细胞中表达显著降低，并与端粒酶功能障碍相关。
2. **文献名称**：Functional analysis of the H/ACA RNP assembly factor NOP10 using targeted antibodies
**作者**：Dragon F, et al.
**摘要**：文章报道了针对NOP10蛋白的多克隆抗体制备及其在复合体组装研究中的应用。实验表明，抗体可特异性识别内源性NOP10.并用于免疫共沉淀验证其与DKC1、GAR1的相互作用，为复合体功能研究提供工具。
3. **文献名称**：NOP10 overexpression correlates with poor prognosis in hepatocellular carcinoma
**作者**：Wong JMY, et al.
**摘要**：研究利用NOP10抗体检测肝癌组织中蛋白表达水平，发现NOP10高表达与肿瘤进展和端粒酶活性升高相关，提示其作为潜在预后标志物的可能性。
4. **文献名称**：Antibody-based profiling of telomerase-associated proteins reveals NOP10 as a critical component
**作者**：Meier UT, et al.
**摘要**：通过多种抗体(包括NOP10抗体)的蛋白质组学分析，系统鉴定了端粒酶复合体的核心组分，证实NOP10在维持复合体结构及rRNA修饰中的必要性。
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**注意**：以上文献为示例性质，实际引用时需通过学术数据库(如PubMed)核实真实文献。建议检索关键词："NOP10 antibody"、"NOP10 AND H/ACA complex" 或结合具体研究领域(如癌症、遗传病)。
NOP10 (Nucleolar Protein 10) is a conserved core component of the H/ACA small nucleolar ribonucleoprotein (snoRNP) complex, which plays a critical role in ribosomal RNA (rRNA) processing and modification, particularly in guiding site-specific pseudouridylation. This post-transcriptional modification is essential for ribosome biogenesis, ensuring proper rRNA folding and stability. NOP10 interacts with other core proteins, including dyskerin (DKC1), GAR1. and NHF2. to stabilize the complex and facilitate its recruitment to target RNAs. Mutations in NOP10 or related H/ACA complex components are linked to genetic disorders such as dyskeratosis congenita, characterized by bone marrow failure and cancer predisposition, highlighting its biological significance.
Antibodies targeting NOP10 are vital tools for studying its expression, localization, and functional roles in cellular processes. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate NOP10’s involvement in ribosome assembly, telomerase RNA maturation, and its dysregulation in diseases. Research-grade anti-NOP10 antibodies are typically raised in rabbits or mice using recombinant protein fragments or synthetic peptides. Validation includes specificity testing via knockdown/knockout controls and functional assays. These reagents help unravel molecular mechanisms underlying ribosomopathies, cancer, and aging-related pathologies, making NOP10 antibodies essential for both basic and translational research.
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