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产品描述：人Ⅰ型胶原N末端肽(NTX)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液样本中的NTX含量。试剂盒检测范围为7.8 nM BCE-500 nM BCE，在骨质疏松症、骨转移癌等骨骼代谢异常相关研究中具有重要价值；适用于骨代谢机制研究、抗骨质疏松药物开发评估、骨骼疾病动物模型分析等科研领域；支持研究者灵活选择体液样本进行横向比对实验；为骨代谢相关基础研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：cross linked N-telopeptide of type 1 collagen,NTX,_x000D_ cross linked N-telopeptide of type I collagen,NTX
缩写：NTX
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine
检测范围：7.8 nM BCE-500 nM BCE
灵敏度：1.95 nM BCE
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NTX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-93 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 92-99 | |||||
回收率：
| The recovery of human NTX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 85-106 | ||||
| EDTA plasma (n=4) | 92 | 88-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| BCEOD1OD2AverageCorrected 5001.9831.9761.9801.850 2501.4461.5501.4981.368 1251.0111.0931.0520.922 62.50.6160.6250.6210.491 31.250.3740.3610.3680.238 15.60.2720.2740.2730.143 7.80.2040.2150.2100.080 00.1310.1290.130 | ||||||
货期：3-5 working days
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