首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠神经降压素(NTS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的NTS含量。大鼠神经降压素（NTS）是一种神经肽，在中枢神经系统中发挥重要作用，参与调节进食、快感、血压等生理过程。其作用机制涉及与特定受体结合，影响神经元活动，进而调节相关生理功能。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于探究神经系统功能、能量代谢调控、肠道生理机制及应激相关疾病模型的分子机制研究，可满足体外培养细胞、动物组织或体液样本的检测需求，为科研工作者提供稳定可靠的数据支持，助力神经生物学、药理学及基础医学领域的探索性研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：NtsNeurotensin/neuromedin N [Cleaved into: Large neuromedin N ELISA Kit; NmN-125); Neuromedin N ELISA Kit; NN ELISA Kit; NmN); Neurotensin ELISA Kit; NT); Tail peptide] ELISA Kit
缩写：NTS
Uniprot No.：P20068
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat NTS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
| 1:2 | Average % | 84 | |||||
| Range % | 80-88 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 90-99 | ||||||
| 1:8 | Average % | 89 | |||||
| Range % | 85-93 | ||||||
回收率：
| The recovery of rat NTS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 97 | 92-103 | |||||
| EDTA plasma (n=4) | 100 | 95-108 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected10002.5412.3792.4602.2535001.8391.7211.7801.5732501.1521.2051.1790.9721250.7850.8160.8010.59462.50.5030.4820.4930.28631.20.3480.3280.3380.13115.60.2940.3030.2990.09200.2030.2100.207 | |||||||
货期：3-5 working days
×
