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Human Neurotensin,NT ELISA Kit

  • 中文名： 人神经降压素(NT)ELISA Kit
货号: IPD-E09144h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人神经降压素/神经调节素N (NTS) 酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NTS含量。NTS即神经降压素（Neurotensin），它是一种脑肠肽，分布于中枢神经系统和胃肠道。在研究机制方面，NTS通过与神经降压素受体结合，参与调节多种生理过程，如痛觉感受、体温调节、内分泌等，其在神经精神疾病及肿瘤等研究中受关注。试剂盒检测范围为15.6 pg/ml- 1000pg/ml，为神经生物学、消化系统疾病研究及药物开发等领域提供可靠工具，支持科研人员探究NT在帕金森病、肠易激综合征等疾病模型中的表达规律，或评估实验干预对NT信号通路的影响。适用于批量样本的高通量检测，为体外实验研究提供高效解决方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Human proneurotensin ELISA Kit; Large neuromedin N ELISA Kit; Neuromedin N preproprotein ELISA Kit; Neurotensin/neuromedin N ELISA Kit; NEUT_HUMAN ELISA Kit; NMN 125 ELISA Kit; NmN ELISA Kit; NmN-125 ELISA Kit; NN ELISA Kit; NT ELISA Kit; NT/N ELISA Kit; NTRH ELISA Kit; NTS ELISA Kit; NTS1 ELISA Kit; Pro neurotensin/neuromedin ELISA Kit; Proneuromedin N mRNA ELISA Kit; Tail peptide ELISA Kit
  • 缩写：
    NTS
  • Uniprot No.：
    P30990
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/ml - 1000pg/ml
  • 灵敏度：
    3.9 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.










  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human NT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    90


    Range %

    85-100


    1:2

    Average %

    94


    Range %

    87-98


    1:4

    Average %

    90


    Range %

    85-96


    1:8

    Average %

    94


    Range %

    90-98









  • 回收率：

    The recovery of human NT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    90

    88-94


    EDTA plasma (n=4)

    90

    85-96
















  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    rId1

     




































































    pg/ml

    OD1

    OD2

    Average

    Corrected


    1000

    2.511

    2.698

    2.605

    2.494


    500

    2.276

    2.305

    2.291

    2.180


    250

    1.848

    1.883

    1.866

    1.755


    125

    1.362

    1.386

    1.374

    1.263


    62.5

    0.781

    0.798

    0.790

    0.679


    31.25

    0.402

    0.432

    0.417

    0.306


    15.6

    0.246

    0.258

    0.252

    0.141


    0

    0.112

    0.109

    0.111




  • 货期：
    3-5 working days

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