首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠N端前脑钠素(NT-proBNP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NT-proBNP含量。NT-proBNP是脑钠肽原裂解后无活性的N末端片段。其分泌受心室壁张力和容量负荷影响。临床上常作为心力衰竭诊断和预后评估的重要生物标志物，通过检测血液中水平，反映心脏功能状态，辅助判断病情严重程度与指导治疗。试剂盒检测范围为7.8 pg/mL-500 pg/mL，适用于心血管疾病机制研究、心肌损伤动物模型评估及药物干预效果分析等科研领域；支持对大鼠心脏组织或循环系统中的NT - proBNP水平进行动态监测，为心肌肥厚、心力衰竭等模型的病理生理研究提供可靠工具，亦可用于探索心血管相关药物对心室功能的调节作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：NT-proBNP
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat NT-proBNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 84-95 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-101 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 94-105 | |||||
| 1:8 | Average % | 84 | ||||
| Range % | 80-88 | |||||
回收率：
| The recovery of rat NT-proBNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-94 | ||||
| EDTA plasma (n=4) | 94 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.7692.6512.7102.5482501.9731.9011.9371.7751251.1831.2171.2001.03862.50.7930.8410.8170.65531.20.5060.5500.5280.36615.60.4040.3790.3920.2307.80.2610.2450.2530.09100.1650.1590.162 | ||||||
货期：3-5 working days
×
