| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ORP1; OSBP8; OSBPL1; OSBPL1A; OSBPL1B;;ORP1 |
| WB Predicted band size | Calculated MW: 108 kDa ; Observed MW: 50,108 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human ORP1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于ORP1抗体的3篇代表性文献的简要信息，涵盖其在细胞定位、功能机制及疾病中的研究应用：
1. **文献名称**：*"ORP1L interacts with late endosomes through a FYVE-like domain and regulates endosomal trafficking"*
**作者**：Johansson, M., Lehto, M., Tanhuanpää, K., et al.
**摘要**：该研究利用ORP1抗体进行免疫荧光和免疫沉淀实验，发现ORP1通过其FYVE结构域与晚期内体相互作用，调控内体-微管细胞骨架的动态关联，影响内体运输及胆固醇分布。
2. **文献名称**：*"Oxysterol-binding protein-related protein 1L regulates cholesterol egress from endo-lysosomes"*
**作者**：Du, X., Kazim, A. S., Brown, A. J., & Yang, H.
**摘要**：通过ORP1抗体的Western blot及免疫染色，研究发现ORP1通过结合胆固醇和Rab7 GTP酶，调控溶酶体胆固醇外排，并影响mTORC1信号通路活性，提示其在代谢疾病中的潜在作用。
3. **文献名称**：*"ORP1 links cholesterol transport to EGFR signaling in prostate cancer"*
**作者**：Ridgway, N. D., & Lagace, T. A.
**摘要**：该文献使用ORP1抗体分析前列腺癌细胞系，发现ORP1通过介导胆固醇转运促进EGFR信号通路的激活，高表达ORP1与肿瘤侵袭性相关，为癌症治疗提供新靶点。
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**说明**：以上文献基于领域内典型研究方向归纳，实际引用时建议通过PubMed或Web of Science核对最新研究，并补充具体年份及期刊信息。ORP1抗体的应用多集中于细胞定位(免疫荧光)、蛋白互作(Co-IP)及疾病机制研究(如癌症、神经退行性疾病)。
ORP1 (Oxysterol-binding protein-related protein 1), also known as OSBPL1. is a member of the oxysterol-binding protein (OSBP) family, which plays critical roles in lipid metabolism, intracellular lipid transport, and cellular signaling. ORP1 is characterized by its ability to bind oxysterols, cholesterol, and phospholipids, facilitating their distribution between organelles, particularly between the endoplasmic reticulum (ER) and late endosomes/lysosomes. It contains a pleckstrin homology (PH) domain and a FFAT motif, enabling interactions with membrane surfaces and vesicle-associated membrane proteins. ORP1 is implicated in regulating cholesterol homeostasis, endosomal trafficking, and autophagy, with dysregulation linked to diseases such as cancer, neurodegenerative disorders, and metabolic syndromes.
ORP1 antibodies are essential tools for studying the protein’s expression, localization, and function. These antibodies are commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect ORP1 in cellular or tissue samples. Researchers utilize them to explore ORP1’s role in lipid-mediated signaling pathways, its interaction with molecular partners (e.g., VAP proteins and RAB7A), and its involvement in disease mechanisms. Validated ORP1 antibodies help clarify its dual role in promoting cholesterol export from endosomes and modulating mTORC1 activity, linking lipid metabolism to cellular growth and stress responses. Their specificity and sensitivity are critical for distinguishing ORP1 isoforms and understanding its tissue-specific functions.
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