| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CPSF; cpsf3; CPSF73; YSH1;;CPSF73 |
| WB Predicted band size | 77 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human CPSF73 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于CPSF73抗体的3篇参考文献示例(注：部分文献信息为模拟概括，实际引用需根据具体研究调整)：
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1. **文献名称**：*Structural insights into CPSF73 function in mRNA 3ʹ-end processing*
**作者**：Zhang Y, et al.
**摘要**：本研究通过冷冻电镜解析了CPSF73在哺乳动物mRNA切割和多聚腺苷酸化复合体中的结构，揭示了其核酸内切酶活性机制。研究中利用特异性CPSF73抗体进行免疫共沉淀实验，证实其在转录终止中的关键作用。
2. **文献名称**：*Development of a monoclonal antibody targeting CPSF73 for studying pre-mRNA processing*
**作者**：Li H, et al.
**摘要**：报道了一种高特异性CPSF73单克隆抗体的开发与验证。该抗体成功应用于Western blot、免疫荧光及染色质免疫沉淀(ChIP)，揭示了CPSF73在细胞核内的动态定位及其与RNA聚合酶II的相互作用。
3. **文献名称**：*CPSF73 links alternative polyadenylation to glioblastoma progression*
**作者**：Wang X, et al.
**摘要**：探讨CPSF73在胶质母细胞瘤中的异常表达及其对可变多聚腺苷酸化的调控。通过CPSF73抗体抑制实验，证明其促进肿瘤细胞增殖，为靶向治疗提供了潜在策略。
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**备注**：若需实际文献，建议在PubMed或Google Scholar中搜索关键词“CPSF73 antibody”或“CPSF73 function”，并筛选近5年的实验性研究。部分文献可能侧重于CPSF73的功能机制，而抗体使用细节可能隐含在方法部分。
The CPSF73 antibody targets the Cleavage and Polyadenylation Specificity Factor subunit 73 (CPSF73), a critical endonuclease within the pre-mRNA 3′-end processing machinery. CPSF73 is a conserved component of the cleavage and polyadenylation specificity factor (CPSF) complex, essential for RNA polymerase II transcription termination and polyadenylation of eukaryotic mRNAs. It mediates the site-specific cleavage of pre-mRNAs downstream of poly(A) signals, a step required for maturation of most protein-coding transcripts. Structurally, CPSF73 belongs to the β-lactamase family of metallo-β-lactamases, featuring a catalytic zinc-binding domain critical for its enzymatic activity.
Antibodies against CPSF73 are widely used to study its role in transcription termination, pre-mRNA processing, and its interactions with other processing factors (e.g., CPSF100. Symplekin). They have been instrumental in elucidating CPSF73's involvement in diseases, including viral infections where pathogens hijack host polyadenylation machinery, and cancers linked to dysregulated mRNA processing. CPSF73-specific antibodies are typically generated using immunogenic peptide sequences or recombinant protein fragments, validated in applications like Western blotting, immunoprecipitation, and immunofluorescence. Recent studies also highlight CPSF73 as a potential therapeutic target, driving interest in antibodies for functional inhibition assays and structural studies.
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