Human Neurogranin(NRGN) ELISA kit

货号: IPD-EL016081HU

Price： ￥2500

48T 96T

数量：

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产品描述：
人神经颗粒素(NRGN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测样本中的NRGN含量。NRGN（神经颗粒素）是神经元特异性大鼠RC3/neurogranin基因的人类同系物，编码一种突触后蛋白激酶底物，在缺乏钙离子的情况下与钙调蛋白结合。其表达受甲状腺激素调控，可能与甲状腺功能减退症对精神状态的后果有关。研究显示，NRGN在突触传递、神经发育和神经退行性疾病中起重要作用。适用于神经生物学研究、疾病模型构建、药物作用机制探索等科研领域，为探究NRGN在神经系统疾病中的功能提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Protein kinase C substrate RC3 ELISA Kit; Calmodulin binding protein ELISA Kit; Hng ELISA Kit; NEUG(55-78) ELISA Kit; NEUG_HUMAN ELISA Kit; Neurogranin (protein kinase C substrate) ELISA Kit; Ng ELISA Kit; NRGN ELISA Kit; Protein kinase C substrate ELISA Kit; RC3 ELISA Kit
缩写：
NRGN
Uniprot No.：
Q92686
种属：
Homo sapiens (Human)
样本类型：
检测范围：
灵敏度：
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Neuroscience
测定原理：
quantitative
测定方法：
Sandwich
本试剂盒所含材料：

A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human NRGN antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled NRGN antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate

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