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产品描述：人神经调节蛋白4(NRG4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NRG4含量。人神经调节蛋白4（NRG4）是一种在神经系统、心脏、乳腺等组织和器官中发挥作用的表皮生长因子，其通过与ErbB4受体结合，激活下游PI3K/Akt、STAT5等信号通路，促进细胞增殖、抑制细胞凋亡和改善细胞能量代谢。NRG4在肥胖、糖尿病、心血管疾病等多种疾病中发挥重要作用，成为潜在的治疗靶点。试剂盒检测范围为125 pg/mL-8000 pg/mL，本产品适用于基础科研中对NRG4在疾病模型、细胞实验或动物组织中的表达水平分析，例如探究代谢相关信号通路机制或药物干预效果评估，为代谢性疾病研究提供可靠的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：DKFZp779N0541 ELISA Kit; DKFZp779N1944 ELISA Kit; Heregulin 4 ELISA Kit; HRG4 ELISA Kit; Neuregulin 4 ELISA Kit; Neuregulin-4 ELISA Kit; NRG-4 ELISA Kit; NRG4 ELISA Kit; NRG4_HUMAN ELISA Kit; pro-neuregulin-4; membrane-bound isoform ELISA Kit; Pro-NRG4 ELISA Kit
缩写：NRG4
Uniprot No.：Q8WWG1
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NRG4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-101 | |||||
| 1:8 | Average % | 85 | ||||
| Range % | 82-89 | |||||
回收率：
| The recovery of human NRG4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-96 | ||||
| EDTA plasma (n=4) | 98 | 94-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected80002.3352.2562.2962.10240001.6481.7681.7081.51420001.1911.2011.1961.00210000.8120.8230.8180.6245000.5880.5960.5920.3982500.4750.4840.4800.2861250.3670.3810.3740.18000.1930.1950.194 | ||||||
货期：3-5 working days
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