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产品描述：人核受体亚家族4A组成员2(NR4A2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的NR4A2含量。NR4A2 属于核受体超家族成员。它在神经保护、代谢调节、肿瘤发生等方面发挥重要作用。研究机制上，其能通过调节下游基因表达参与多种生理过程，异常表达或功能失调与神经退行性疾病、癌症等多种疾病的发生发展密切相关。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，科研人员可通过深入研究NR4A2在神经系统疾病中的分子机制，探索其在肿瘤微环境调控中的作用，或评估炎症相关信号通路中该蛋白的动态变化，为神经生物学、肿瘤学及免疫学等领域的基础研究提供有效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HZF 3 ELISA Kit; HZF3 ELISA Kit; Immediate-early response protein NOT ELISA Kit; Intermediate early receptor protein ELISA Kit; NGFI B/nur77 beta type transcription factor homolog ELISA Kit; NOT ELISA Kit; Nr4a2 ELISA Kit; NR4A2_HUMAN ELISA Kit; nuclear receptor of T cells ELISA Kit; nuclear receptor related 1 ELISA Kit; Nuclear receptor subfamily 4 group A member 2 ELISA Kit; Nur related protein 1 homolog ELISA Kit; nur related protein-1; human homolog of ELISA Kit; Nurr 1 ELISA Kit; Orphan nuclear receptor NR4A2 ELISA Kit; Orphan nuclear receptor NURR1 ELISA Kit; RNR 1 ELISA Kit; RNR1 ELISA Kit; T cell nuclear receptor NOT ELISA Kit; T-cell nuclear receptor NOT ELISA Kit; TINUR ELISA Kit; Transcriptionally inducible nuclear receptor ELISA Kit; Transcriptionally inducible nuclear receptor related ELISA Kit; Transcriptionally inducible nuclear receptor related 1 ELISA Kit; Transcriptionally-inducible nuclear receptor ELISA Kit
缩写：NR4A2
Uniprot No.：P43354
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NR4A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 89-101 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 86-106 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 90-103 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 82-102 | |||||
回收率：
| The recovery of human NR4A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 86-96 | ||||
| EDTA plasma (n=4) | 93 | 86-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.0842.0982.0911.98810001.7671.7861.7771.6745001.3591.3221.3411.2382500.8170.8280.8230.7201250.5210.5340.5280.42562.50.3400.3320.3360.23331.250.2850.2740.2800.17700.1040.1010.103 | ||||||
货期：3-5 working days
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