首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人糖皮质激素受体(NR3C1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的NR3C1含量。NR3C1是重要的药物靶点。它编码糖皮质激素受体，在细胞生长、分化和代谢等生理过程中起关键作用。研究机制主要围绕其与糖皮质激素结合后调节基因表达，影响多种信号通路，在炎症、自身免疫病等方面研究较多。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于科研场景中探索糖皮质激素信号通路的调控机制；可用于体外研究糖皮质激素受体在炎症模型、代谢疾病动物实验或细胞应激反应中的动态变化，为分子机制研究及药物干预效果评价提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：GCCR ELISA Kit; GCR ELISA Kit; GCR_HUMAN ELISA Kit; GCRST ELISA Kit; glucocorticoid nuclear receptor variant 1 ELISA Kit; Glucocorticoid receptor ELISA Kit; GR ELISA Kit; GRL ELISA Kit; Grl1 ELISA Kit; nr3c1 ELISA Kit; Nuclear receptor subfamily 3 group C member 1 ELISA Kit; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) ELISA Kit
缩写：NR3C1
Uniprot No.：P04150
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NR3C1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 82-95 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 87-99 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 82-92 | |||||
回收率：
| The recovery of human NR3C1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 92-102 | ||||
| EDTA plasma (n=4) | 106 | 96-110 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20001.7241.7421.7331.60010001.1971.1761.1871.0545000.7630.7530.7580.6252500.3910.3860.3890.2561250.3060.2870.2970.16462.50.2360.2440.2400.10731.250.1890.1790.1840.05100.1320.1340.133 | ||||||
货期：3-5 working days
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