首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠糖皮质类固醇受体(GR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的NR3C1含量。NR3C1 即糖皮质激素受体，在体内广泛分布。它与糖皮质激素结合后入核调控基因转录，参与众多生理过程如免疫、代谢等。在研究中，它是多种疾病如炎症、自身免疫病、肿瘤等的潜在靶点，探索其机制对开发新疗法意义重大。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于科研领域探索应激反应机制、评估药物干预对GR表达的影响，或结合病理模型研究GR在组织特异性调控中的作用，为神经内分泌、免疫代谢等基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Nr3c1 ELISA Kit; GrlGlucocorticoid receptor ELISA Kit; GR ELISA Kit; Nuclear receptor subfamily 3 group C member 1 ELISA Kit
缩写：NR3C1
Uniprot No.：P06536
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat GR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-98 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 93-105 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 92-100 | |||||
回收率：
| The recovery of rat GR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 88-97 | ||||
| EDTA plasma (n=4) | 84 | 80-89 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.5972.6572.6272.5075002.0192.1062.0631.9432501.3761.4091.3931.2731250.9090.8420.8760.75662.50.5660.5240.5450.42531.20.3410.3210.3310.21115.60.2280.2440.2360.11600.1230.1170.120 | ||||||
货期：3-5 working days
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