首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠肾病蛋白(nephrin)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NPHS1含量。NPHS1 是一个重要靶点，它与肾脏疾病紧密相关，其编码的蛋白参与肾小球滤过屏障的构成。研究发现，NPHS1 突变可导致先天性肾病综合征。学者们正深入研究其作用机制，期望为相关肾病的诊断和治疗带来新策略。试剂盒检测范围为1.25 ng/mL-80 ng/mL，适用于科研领域中对肾小球足细胞相关功能的研究；适用于体外研究肾脏疾病模型中足细胞损伤机制、药物对肾小球结构影响的评估，或通过检测组织匀浆探索肾小球发育及病理改变相关的分子机制本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Nphs1 ELISA Kit; Nphn ELISA Kit; Nephrin ELISA Kit; Renal glomerulus-specific cell adhesion receptor ELISA Kit
缩写：NPHS1
Uniprot No.：Q9R044
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.25 ng/mL-80 ng/mL
灵敏度：0.312 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Nephrin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-98 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 81-89 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 97-103 | |||||
回收率：
| The recovery of rat Nephrin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 101 | 97-105 | ||||
| EDTA plasma (n=4) | 98 | 95-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected802.2152.1942.2052.049401.8461.8041.8251.669201.4441.4681.4561.300101.0331.0681.0510.89550.6340.6710.6530.4972.50.3730.3980.3860.2301.250.2970.2980.2980.14200.1540.1570.156 | ||||||
货期：3-5 working days
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