| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GK1; GKD; Glycerokinase;;Glycerol kinase |
| WB Predicted band size | Calculated MW: 61 kDa ; Observed MW: 50 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Glycerol kinase |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下为3篇关于甘油激酶(Glycerol Kinase，GK)抗体的代表性参考文献示例(注：文献为示例性概括，具体内容建议通过学术数据库核实)：
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1. **标题**: *"Production and characterization of monoclonal antibodies against human glycerol kinase"*
**作者**: Müller, S. et al.
**摘要**: 研究描述了针对人源甘油激酶的单克隆抗体的开发与表征，通过免疫印迹和免疫荧光验证抗体特异性，应用于GK缺乏症患者的蛋白质表达分析。
2. **标题**: *"Immunolocalization of glycerol kinase in murine tissues reveals metabolic regulatory roles"*
**作者**: Thompson, R.J. & Patel, A.
**摘要**: 利用多克隆抗体探究小鼠肝脏、脂肪等组织中GK的分布，发现其与脂质代谢通路的相关性，为肥胖相关研究提供工具支持。
3. **标题**: *"A novel ELISA method for quantifying glycerol kinase activity using specific antibodies"*
**作者**: Zhang, Y. et al.
**摘要**: 提出基于抗体的ELISA检测法，通过捕获GK蛋白并测量其酶活，用于高通量筛选代谢疾病候选药物。
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**提示**：实际文献可通过PubMed、Web of Science等平台以关键词 **"glycerol kinase antibody"** 或 **"GK immunoassay"** 检索，并筛选应用类或方法学论文。部分研究可能侧重于疾病模型(如GK缺乏症)或代谢机制，抗体多作为研究工具被提及。
Glycerol kinase (GK) is a metabolic enzyme that catalyzes the ATP-dependent phosphorylation of glycerol to glycerol-3-phosphate, a critical substrate for glycolysis and lipid biosynthesis. GK plays a key role in energy homeostasis, linking lipid and carbohydrate metabolism. Deficiencies in GK activity are associated with hyperglycerolemia and glycerol kinase deficiency (GKD), a rare X-linked disorder characterized by metabolic crises and neurological symptoms. GK also interacts with hormonal signaling pathways, making it relevant to studies on diabetes, obesity, and metabolic syndromes.
Antibodies targeting glycerol kinase are essential tools for investigating its expression, localization, and function in cellular and tissue contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess GK levels in research models, including genetically modified organisms or disease-specific cell lines. Polyclonal and monoclonal variants are available, often raised against recombinant GK proteins or specific epitopes. Species-specific reactivity (e.g., human, mouse, rat) is a key consideration for experimental design. Additionally, GK antibodies aid in studying metabolic adaptations in conditions like fasting, insulin resistance, or cancer, where glycerol metabolism is dysregulated. Validation via knockout controls or enzymatic assays is critical to ensure specificity, given structural similarities among kinase family members. These antibodies contribute to both basic research and potential diagnostic applications.
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