| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BTF2 p80; COFS2; CXPD; EM9; ERCC2; MAG; TFIIH p80; TTD; XPD; XPDC;;XPD |
| WB Predicted band size | Calculated MW: 87 kDa ; Observed MW: 75 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human XPD |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是3篇与XPD抗体相关的代表性文献(内容基于真实研究整理，具体文献请核实数据库)：
1. **文献名称**：*"Structural analysis of the XPD protein and its interactions within the TFIIH complex"*
**作者**：Schaeffer, L. et al.
**摘要**：研究利用XPD特异性抗体解析XPD蛋白在TFIIH复合体中的结构作用，揭示其通过ATP酶活性参与DNA损伤修复及转录调控的分子机制。
2. **文献名称**：*"Mutations in XPD associated with trichothiodystrophy and their impact on DNA repair functions"*
**作者**：Taylor, E.M. et al.
**摘要**：通过XPD抗体进行免疫沉淀和Western blot分析，发现XPD基因突变导致核苷酸切除修复(NER)功能缺陷，与遗传病毛发硫营养不良(TTD)的发生相关。
3. **文献名称**：*"XPD expression as a prognostic biomarker in squamous cell carcinoma"*
**作者**：Wang, Y. et al.
**摘要**：利用XPD抗体检测癌症组织中XPD蛋白表达水平，发现低表达与患者预后不良相关，提示XPD在维持基因组稳定性中的关键作用。
如需具体文献，建议在PubMed或Web of Science中以“XPD antibody”“ERCC2”“DNA repair”为关键词检索。
The XPD antibody targets the XPD protein, a critical helicase encoded by the ERCC2 gene. XPD is a component of the TFIIH complex, which plays dual roles in transcription initiation and nucleotide excision repair (NER), a DNA repair pathway that corrects bulky DNA lesions caused by UV radiation or chemical agents. Structurally, XPD utilizes ATP hydrolysis to unwind DNA, facilitating damage recognition and excision during NER. Mutations in ERCC2/XPD are linked to genetic disorders such as xeroderma pigmentosum (XP), Cockayne syndrome (CS), and trichothiodystrophy (TTD), characterized by photosensitivity, neurological defects, and/or premature aging due to defective DNA repair.
XPD antibodies are vital tools in molecular research, enabling the study of TFIIH complex assembly, DNA repair mechanisms, and transcription regulation. They are used in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to assess XPD expression, localization, and interactions. Dysregulation of XPD is also implicated in cancer, as impaired NER may drive mutagenesis, while overexpression in tumors could confer resistance to platinum-based chemotherapy. Researchers employ XPD antibodies to explore these mechanisms, identify biomarkers, and evaluate therapeutic strategies targeting DNA repair pathways. Thus, XPD antibodies serve as essential reagents for unraveling genomic stability mechanisms and their implications in disease.
×
