首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人神经型一氧化氮合成酶(nNOS)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的NOS1含量。NOS1即神经元型一氧化氮合酶，在神经系统中分布广泛。它可催化L -精氨酸生成一氧化氮（NO），NO作为信号分子参与神经传递、学习记忆等生理过程。其研究机制聚焦于NO的产生调控及在神经疾病如阿尔茨海默病等中的作用。试剂盒检测范围为0.156 IU/mL-10 IU/mL，适用于基础科研领域，例如研究神经系统疾病模型中的酶活性变化、评估药物或基因干预对nNOS表达的影响，也可用于体外培养神经元或胶质细胞的功能机制探索，为神经科学、药理学及分子生物学研究提供可靠的工具支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：2310005C01Rik ELISA Kit; BNOS ELISA Kit; Constitutive NOS ELISA Kit; EC 1.14.13.39 ELISA Kit; IHPS 1 ELISA Kit; IHPS1 ELISA Kit; N-NOS ELISA Kit; NC-NOS ELISA Kit; neuronal Nitric Oxide Synthase ELISA Kit; Neuronal NOS ELISA Kit; Nitric oxide synthase ; neuronal; included ELISA Kit; Nitric oxide synthase 1 (neuronal) ELISA Kit; Nitric oxide synthase 1 ELISA Kit; Nitric oxide synthase; brain ELISA Kit; Nitric oxide synthase; penile neuronal; included ELISA Kit; NNOS ELISA Kit; NO ELISA Kit; NOS 1 ELISA Kit; NOS ELISA Kit; NOS type I ELISA Kit; NOS-I ELISA Kit; NOS1 ELISA Kit; NOS1_HUMAN ELISA Kit; Peptidyl-cysteine S-nitrosylase NOS1 ELISA Kit
缩写：NOS1
Uniprot No.：P29475
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：0.156 IU/mL-10 IU/mL
灵敏度：0.039 IU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human nNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 87 | ||||
| Range % | 82-90 | |||||
| 1:10 | Average % | 105 | ||||
| Range % | 101-110 | |||||
| 1:20 | Average % | 83 | ||||
| Range % | 80-86 | |||||
| 1:40 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human nNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-93 | ||||
| EDTA plasma (n=4) | 100 | 95-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| IU/mlOD1OD2AverageCorrected102.9022.9632.9332.74352.3132.3652.3392.1492.51.4691.4651.4671.2771.250.8780.8220.8500.6600.6250.5680.5450.5570.3670.3120.4070.4350.4210.2310.1560.3140.3260.3200.13000.1920.1870.190 | ||||||
货期：3-5 working days
×
