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产品描述：人核苷二磷酸激酶A(NME1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的NME1含量。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，该产品适用于肿瘤生物学研究、细胞代谢调控分析、疾病相关分子机制探索等方向，尤其为肿瘤转移抑制、基因表达谱分析及药物靶点筛选提供定量支持。所有应用仅限于科学研究范畴。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AWD ELISA Kit; AWD, drosophila, homolog of ELISA Kit; GAAD ELISA Kit; Granzyme A activated DNase ELISA Kit; Granzyme A-activated DNase ELISA Kit; GZMA activated DNase ELISA Kit; Metastasis inhibition factor NM23 ELISA Kit; NB ELISA Kit; NBS ELISA Kit; NDK A ELISA Kit; NDKA ELISA Kit; NDKA_HUMAN ELISA Kit; NDP kinase A ELISA Kit; NDPK-A ELISA Kit; NDPKA ELISA Kit; NM23 ELISA Kit; NM23 long variant, included ELISA Kit; nm23-H1 ELISA Kit; NM23-M1 ELISA Kit; NM23H1B, included ELISA Kit; NME/NM23 nucleoside diphosphate kinase 1 ELISA Kit; Nme1 ELISA Kit; NME1-NME2 spliced read-through transcript, included ELISA Kit; Non-metastatic cells 1, protein (NM23A) expressed in ELISA Kit; Nonmetastatic cells 1, protein expressed in ELISA Kit; Nonmetastatic protein 23 ELISA Kit; Nonmetastatic protein 23, homolog 1 ELISA Kit; Nucleoside diphosphate kinase A ELISA Kit; Tumor metastatic process-associated protein ELISA Kit
缩写：NME1
Uniprot No.：P15531
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.68 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NME1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-90 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 84-105 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 80-100 | |||||
回收率：
| The recovery of human NME1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 81-98 | ||||
| EDTA plasma (n=4) | 98 | 90-110 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected12002.1122.0522.0821.9316001.8491.7061.7781.6273001.4931.4211.4571.3061501.0210.9931.0070.856750.5780.5540.5660.41537.50.3420.3150.3290.17818.750.2310.2190.2250.07400.1550.1470.151 | ||||||
货期：3-5 working days
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