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Human NACHT, LRR and PYD domains-containing protein 3

  • 中文名： 人隐热蛋白(NLRP3/C1orf7/CIAS1/NALP3/PYPAF1) ELISA kit
货号: IPD-E15885h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人隐热蛋白(NLRP3/C1orf7/CIAS1/NALP3/PYPAF1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NLRP3含量。NLRP3是一种细胞内模式识别受体，参与炎症反应。其研究背景涉及多种疾病，如炎症性疾病和自身免疫性疾病。研究机制主要关注NLRP3的激活和调节，以及其与NEK7、ASC和pro-Caspase-1等蛋白的相互作用。试剂盒检测范围为0.156 ng/ml- 10 ng/ml，适用于炎症相关信号通路研究、药物作用机制探索及疾病动物模型评估，为科研人员探究NLRP3在免疫调控、细胞焦亡及慢性炎症中的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    AGTAVPRL ELISA Kit; AII/AVP ELISA Kit; Angiotensin/vasopressin receptor AII/AVP like ELISA Kit; Angiotensin/vasopressin receptor AII/AVP-like ELISA Kit; C1orf7 ELISA Kit; Caterpiller protein 1.1 ELISA Kit; CIAS 1 ELISA Kit; CIAS1 ELISA Kit; CLR1.1 ELISA Kit; Cold autoinflammatory syndrome 1 ELISA Kit; Cold autoinflammatory syndrome 1 protein ELISA Kit; Cryopyrin ELISA Kit; Familial cold autoinflammatory syndrome ELISA Kit; FCAS ELISA Kit; FCU ELISA Kit; LRR and PYD domains-containing protein 3 ELISA Kit; Muckle-Wells syndrome ELISA Kit; MWS ELISA Kit; NACHT ELISA Kit; NACHT LRR and PYD containing protein 3 ELISA Kit; NALP 3 ELISA Kit; NALP3 ELISA Kit; NALP3_HUMAN ELISA Kit; NLR family pyrin domain containing 3 ELISA Kit; NLRP3 ELISA Kit; PYPAF 1 ELISA Kit; PYPAF1 ELISA Kit; PYRIN containing APAF1 like protein 1 ELISA Kit; PYRIN-containing APAF1-like protein 1 ELISA Kit
  • 缩写：
    NLRP3
  • Uniprot No.：
    Q96P20
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/ml - 10 ng/ml
  • 灵敏度：
    0.039 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human NLRP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    87


    Range %

    80-94


    1:2

    Average %

    85


    Range %

    80-89


    1:4

    Average %

    97


    Range %

    94-100


    1:8

    Average %

    95


    Range %

    90-98


  • 回收率：

    The recovery of human NLRP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    94

    90-98


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    10

    2.913

    2.801

    2.857

    2.741


    5

    2.402

    2.303

    2.353

    2.237


    2.5

    1.696

    1.597

    1.647

    1.531


    1.25

    1.122

    1.084

    1.103

    0.987


    0.625

    0.679

    0.667

    0.673

    0.557


    0.312

    0.433

    0.426

    0.430

    0.314


    0.156

    0.267

    0.249

    0.258

    0.142


    0

    0.118

    0.114

    0.116




  • 货期：
    3-5 working days

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