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产品描述：人神经损伤诱导蛋白-1(NINJ1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的NINJ1含量。试剂盒检测范围为25 pg/mL-1600 pg/mL，其宽泛的样本兼容性使其适用于体外培养细胞模型、动物组织样本及生物体液中的NINJ1动态监测，可用于神经退行性疾病模型构建、药物干预效果评估、神经再生机制研究等基础科研领域，为探索阿尔茨海默病、多发性硬化症等疾病的生物标志物提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：NINJ1; Ninjurin-1; Nerve injury-induced protein 1
缩写：NINJ1
Uniprot No.：Q92982
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NINJ1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 105 | |||||
| Range % | 94-109 | ||||||
| 1:2 | Average % | 98 | |||||
| Range % | 90-102 | ||||||
| 1:4 | Average % | 88 | |||||
| Range % | 81-91 | ||||||
| 1:8 | Average % | 107 | |||||
| Range % | 98-111 | ||||||
回收率：
| The recovery of human NINJ1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 94 | 90-98 | |||||
| EDTA plasma (n=4) | 104 | 94-107 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected16002.1652.2432.2042.0608001.7181.7651.7421.5984001.0421.0181.0300.8862000.5870.5790.5830.4391000.3280.3340.3310.187500.2420.2550.2490.105250.1860.1940.1900.04600.1460.1420.144 | |||||||
货期：3-5 working days
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