Human Neuroglobin,NGB ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E08737h

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人脑红蛋白(NGB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NGB含量。NGB即脑红蛋白，作为珠蛋白家族新成员存在于神经系统。研究机制上，它具有可逆结合氧的特性，能提高神经细胞氧的储备与运输；还可在缺氧时发挥细胞保护功能，抵御氧化应激和凋亡，对神经系统正常功能维持意义重大。试剂盒检测范围为0.27 ng/ml- 200 ng/ml，适用于科研场景中脑组织缺氧损伤模型评估、神经退行性疾病机制探索以及药物干预效果分析等实验需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Neuroglobin ELISA Kit; NGB ELISA Kit; NGB_HUMAN ELISA Kit
缩写：
NGB
Uniprot No.：
Q9NPG2
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
0.27 ng/ml - 200 ng/ml
灵敏度：
0.27 ng/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Neuroscience
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human NGB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	89
	Range %	83-96
1:2	Average %	92
	Range %	87-97
1:4	Average %	95
	Range %	91-102
1:8	Average %	99
	Range %	93-105

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
200	2.281	2.436	2.359	2.215
66.6	1.861	1.912	1.887	1.743
22.2	1.201	1.274	1.238	1.094
7.4	0.723	0.776	0.750	0.606
2.46	0.497	0.529	0.513	0.369
0.82	0.382	0.402	0.392	0.248
0.27	0.245	0.234	0.240	0.096
0	0.147	0.141	0.144