首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠脑红蛋白(NGB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的NGB含量。NGB（神经球蛋白）是一种存在于神经元中的携氧蛋白，具有抗氧化应激和神经保护功能。研究显示，NGB通过调节mTOR信号通路促进自噬，从而保护神经元。此外，NGB在神经退行性疾病、脑血管疾病和脑外伤等疾病中发挥重要作用。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，该产品可广泛应用于神经生物学基础研究，如脑损伤模型、缺氧应激实验或神经退行性疾病动物模型中NGB的表达模式探索，也可用于体外细胞培养体系中氧代谢相关机制的研究，为阐明NGB在神经保护或病理进程中的作用提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ngb ELISA Kit; Neuroglobin ELISA Kit
缩写：NGB
Uniprot No.：Q99JA8
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 ng/mL-1000 ng/mL
灵敏度：3.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat NGB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-90 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 96-102 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 90-96 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 84-92 | |||||
回收率：
| The recovery of rat NGB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 104 | 100-108 | ||||
| EDTA plasma (n=4) | 98 | 94-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected10002.2562.1042.1802.0355001.6781.6371.6581.5132501.1311.1681.1501.0051250.7420.7650.7540.60962.50.5100.5000.5050.36031.20.4160.4010.4090.26415.60.3010.3110.3060.16100.1440.1460.145 | ||||||
货期：3-5 working days
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