| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HMG17; HMGN2;;HMGN2 |
| WB Predicted band size | Calculated MW: 9 kDa ; Observed MW: 18 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human HMGN2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是关于HMGN2抗体的模拟参考文献示例(注：以下内容为虚构示例，实际文献需通过学术数据库检索)：
---
1. **文献名称**: *HMGN2 regulates chromatin accessibility and promotes DNA repair in mammalian cells*
**作者**: Zhang L, et al.
**摘要**: 本研究利用HMGN2特异性抗体，通过染色质免疫沉淀(ChIP)和免疫荧光技术，揭示了HMGN2通过调控染色质松弛状态促进DNA损伤修复的分子机制。抗体验证显示其在HeLa细胞中特异性识别HMGN2蛋白。
---
2. **文献名称**: *Antibody-based detection of HMGN2 in inflammatory responses*
**作者**: Kim S, et al.
**摘要**: 通过Western blot和免疫组化分析，研究者发现HMGN2在巨噬细胞炎症反应中表达上调。研究使用商业化HMGN2抗体(货号AB123)，证实其在小鼠模型和人组织样本中的特异性，提示HMGN2可能参与先天免疫调控。
---
3. **文献名称**: *HMGN2 modulates gene transcription in embryonic stem cells*
**作者**: Wang Y, et al.
**摘要**: 本文通过CRISPR/Cas9敲除结合HMGN2抗体介导的染色质分析，证明HMGN2通过维持核心启动子区染色质开放状态，调控多能性基因(如Oct4)的转录，为干细胞分化研究提供新视角。
---
4. **文献名称**: *Development and validation of a polyclonal HMGN2 antibody for cancer research*
**作者**: Gupta R, et al.
**摘要**: 研究团队开发了一种兔源多克隆HMGN2抗体，并通过ELISA、免疫印迹和免疫沉淀验证其高亲和力与特异性。该抗体成功应用于乳腺癌组织芯片分析，提示HMGN2表达与肿瘤分级相关。
---
**提示**：实际研究中建议通过PubMed、Google Scholar等平台检索关键词(如"HMGN2 antibody"、"HMGN2 immunodetection")，并筛选近5年文献以获取最新进展。
**Background of HMGN2 Antibody**
HMGN2 (High Mobility Group Nucleosome-Binding Domain 2), also known as HMG-17. is a member of the high-mobility group (HMG) protein family, which plays a critical role in modulating chromatin structure and regulating gene expression. HMGN2 binds to nucleosomes and facilitates chromatin decompaction, enhancing transcriptional accessibility for DNA-dependent processes. It is involved in epigenetic regulation, DNA repair, and cellular differentiation, making it a key focus in studies related to cancer, development, and chromatin dynamics.
HMGN2 antibodies are essential tools for detecting and quantifying HMGN2 protein levels in various experimental applications, including Western blotting, immunofluorescence, immunohistochemistry (IHC), and chromatin immunoprecipitation (ChIP). These antibodies are typically developed in host species like rabbits or mice using immunogenic peptides or recombinant HMGN2 proteins. Specificity is validated through knockout controls or siRNA-mediated knockdown to ensure minimal cross-reactivity with other HMG family members.
Research utilizing HMGN2 antibodies has contributed to understanding its role in diseases, such as cancer progression (via transcriptional regulation of oncogenes/tumor suppressors) and inflammatory responses. Commercial HMGN2 antibodies are available from multiple suppliers, often accompanied by validation data in specific applications. Proper validation remains critical due to potential variability in antibody performance across experimental conditions.
×
