首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠核因子-κB亚基p65亲和肽(NF-κB p65)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NFκB p65含量。NFκB p65是重要的转录因子，在细胞的炎症、免疫、增殖和凋亡等多种生理病理过程中发挥关键作用。其激活机制多样，与多条信号通路相互关联。对其研究有助于深入了解疾病发病机制，为开发针对性疗法如抗炎、抗肿瘤药物等提供理论基础。试剂盒检测范围为1.56 pg/mL-100 pg/mL，适用于基础科研中炎症机制研究、免疫调控实验或药物干预效果评估，例如在动物模型中分析NF-κB信号通路激活状态，或探究氧化应激、感染性疾病等实验条件下p65亚基的动态表达。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：NF-Kappa B,nuclear factor-kappa B
缩写：NFκB p65
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat NF-κB p65 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 89 | ||||
| Range % | 86-92 | |||||
| 1:200 | Average % | 99 | ||||
| Range % | 97-103 | |||||
| 1:400 | Average % | 89 | ||||
| Range % | 85-93 | |||||
| 1:800 | Average % | 95 | ||||
| Range % | 91-99 | |||||
回收率：
| The recovery of rat NF-κB p65 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-97 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected1002.0392.0222.0311.887501.3341.3261.3301.186250.9080.9020.9050.76112.50.5660.5570.5620.4186.250.3580.3640.3610.2173.120.2910.2810.2860.1421.560.2430.2320.2380.09400.1450.1430.144 | ||||||
货期：3-5 working days
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