| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DOCK 2; IMD40;;Dedicator of cytokinesis protein 2 |
| WB Predicted band size | 212 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Dedicator of cytokinesis protein 2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇与DOCK2抗体相关的模拟参考文献(基于公开研究主题概括，非真实文献)：
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1. **"DOCK2 regulates Rac activation and cytoskeletal reorganization in T cell migration"**
*Author: Fukui Y, et al.*
*摘要*：研究利用DOCK2特异性抗体，通过免疫印迹和免疫荧光实验，证实DOCK2在T细胞中通过激活Rac GTP酶介导趋化因子触发的细胞骨架重排和迁移，为免疫细胞定向运动提供分子机制。
2. **"DOCK2 deficiency impairs dendritic cell-mediated T cell activation in autoimmune disease"**
*Author: Nishikimi A, et al.*
*摘要*：通过DOCK2抗体敲除模型，发现DOCK2缺失导致树突状细胞趋化性和抗原呈递功能缺陷，降低自身免疫性疾病中T细胞活化，提示DOCK2作为潜在治疗靶点。
3. **"Structural basis of DOCK2-mediated Rac GTPase activation in leukocyte trafficking"**
*Author: Côté JF, et al.*
*摘要*：结合X射线晶体学和DOCK2抗体共沉淀实验，解析DOCK2的DHR-2结构域与Rac的相互作用，阐明其催化Rac核苷酸交换的分子机制，为抑制白细胞异常迁移提供结构基础。
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*注：以上文献名为示例性概括，实际引用请通过PubMed或学术数据库查询真实文献。*
DOCK2 (Dedicator of Cytokinesis 2) is a member of the DOCK protein family, which functions as atypical guanine nucleotide exchange factors (GEFs) for Rho GTPases. Specifically, DOCK2 regulates Rac activation, a key process in cytoskeletal reorganization, cell migration, and immune cell signaling. It is predominantly expressed in hematopoietic cells, particularly lymphocytes, and plays a critical role in immune responses. Studies highlight its importance in chemokine-directed trafficking of T cells, B cells, and natural killer (NK) cells, as well as in the activation and function of these cells. DOCK2-deficient mice exhibit impaired immune cell migration and compromised adaptive immunity, underscoring its biological relevance.
The DOCK2 antibody is a research tool designed to detect and quantify DOCK2 protein expression in experimental settings. It is widely used in immunological studies to investigate mechanisms of immune cell migration, immune synapse formation, and intracellular signaling pathways. Applications include Western blotting, immunofluorescence, flow cytometry, and immunohistochemistry. Researchers also employ DOCK2 antibodies to explore its involvement in diseases such as autoimmune disorders, inflammatory conditions, and cancers, where dysregulated Rac signaling or immune cell dysfunction is implicated. Recent interest in DOCK2 as a potential therapeutic target further drives the development and validation of specific antibodies for functional studies and drug discovery.
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