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产品描述：人核因子κB(NF-κB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的NFKB1含量。NFKB1是重要靶点。它在细胞生长、凋亡、免疫应答等生理过程中起关键作用。其异常激活与炎症、肿瘤等疾病发生发展密切相关。研究主要聚焦于NFKB1在信号通路中的调控机制，通过干预其活性有望为相关疾病治疗提供新策略。试剂盒检测范围为0.312 ng/mL-20 ng/mL，本产品可广泛应用于基础研究中的炎症信号通路解析、药物靶点筛选或细胞应激模型评估等场景，为探索NF-κB在疾病发生发展中的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：DKFZp686C01211 ELISA Kit; DNA binding factor KBF1 ELISA Kit; DNA binding factor KBF1 EBP1 ELISA Kit; DNA-binding factor KBF1 ELISA Kit; EBP 1 ELISA Kit; EBP-1 ELISA Kit; EBP1 ELISA Kit; KBF1 ELISA Kit; MGC54151 ELISA Kit; nf b ELISA Kit; NF kappa B ELISA Kit; NF kappaB ELISA Kit; NF kappabeta ELISA Kit; NF kB1 ELISA Kit; NF-kappaB ELISA Kit; NFkappaB ELISA Kit; NFKB 1 ELISA Kit; NFKB p105 ELISA Kit; NFKB p50 ELISA Kit; NFKB-p105 ELISA Kit; Nfkb1 ELISA Kit; NFKB1_HUMAN ELISA Kit; Nuclear factor kappa B DNA binding subunit ELISA Kit; Nuclear factor kappa-B ELISA Kit; Nuclear factor kappa-B, subunit 1 ELISA Kit; Nuclear factor NF kappa B p105 subunit ELISA Kit; Nuclear factor NF kappa B p50 subunit ELISA Kit; Nuclear factor NF-kappa-B p50 subunit ELISA Kit; Nuclear factor of kappa light chain gene enhancer in B cells 1 ELISA Kit; Nuclear factor of kappa light polypeptide gene enhancer in B cells 1 ELISA Kit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 ELISA Kit; p105 ELISA Kit; p50 ELISA Kit; p84/NF-kappa-B1 p98 ELISA Kit; Transcription factor NFKB1 ELISA Kit
缩写：NFKB1
Uniprot No.：P19838
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NF-κB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 85-94 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 91-103 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 87-96 | |||||
回收率：
| The recovery of human NF-κB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-94 | ||||
| EDTA plasma (n=4) | 95 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.8892.7522.8212.710102.2512.1492.2002.08951.3991.3281.3641.2532.50.6540.6430.6490.5381.250.3960.4100.4030.2920.6250.2410.2550.2480.1370.3120.1680.1810.1750.06400.1120.1100.111 | ||||||
货期：3-5 working days
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