首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人神经丝蛋白L (NF-L)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NEFL含量。NEFL 即神经丝轻链，是神经元细胞骨架的重要组成部分。它在神经系统发育和维持神经结构稳定等方面发挥关键作用。研究机制上，其水平变化可反映神经元损伤或神经退行性病变，常作为神经系统疾病的生物标志物用于诊断和病情监测。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于神经系统疾病模型研究、生物标志物筛选、药物作用机制探索等科研场景，为评估神经元损伤程度、追踪疾病进展或治疗效果提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：NEFL; NF68; NFL; Neurofilament light polypeptide; NF-L; 68 kDa neurofilament protein; Neurofilament triplet L protein
缩写：NEFL
Uniprot No.：P07196
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NF-L in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 99 | ||||
| Range % | 91-105 | |||||
| 1:10 | Average % | 85 | ||||
| Range % | 80-92 | |||||
| 1:20 | Average % | 88 | ||||
| Range % | 82-94 | |||||
| 1:40 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human NF-L spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 98 | 92-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.7702.6652.7182.62810002.1142.0062.0601.9705001.2561.2431.2501.1602500.6570.6510.6540.5641250.3430.3390.3410.25162.50.2520.2420.2470.15731.250.2090.1980.2040.11400.0920.0880.090 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human NEFL antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled NEFL antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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