首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人Norrin(NDP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NDP含量。Norrin（NDP）是一种分泌型视网膜生长因子，与X染色体相关的家族性渗出性玻璃体视网膜病变密切相关。它通过激活Wnt信号通路，在视网膜血管生成和神经保护中发挥重要作用。研究显示，Norrin与Fz4受体结合，激活Wnt/beta-catenin通路，并可能参与神经细胞分化和增殖的调节。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于科研领域中对NDP表达水平的定量分析，例如研究眼部发育机制、血管生成调控或相关疾病的分子病理模型构建。支持多种生物样本类型检测，操作简便且重复性良好，可为体外实验提供高精度数据支持，满足基础研究中对NDP蛋白动态变化的监测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：EVR2 ELISA Kit; Exudative vitreoretinopathy 2 (X linked) ELISA Kit; FEVR ELISA Kit; ND ELISA Kit; NDP ELISA Kit; NDP_HUMAN ELISA Kit; Norrie disease (pseudoglioma) ELISA Kit; Norrie disease protein ELISA Kit; Norrin ELISA Kit; Norrin precursor ELISA Kit; X linked exudative vitreoretinopathy 2 protein ELISA Kit; X-linked exudative vitreoretinopathy 2 protein ELISA Kit
缩写：NDP
Uniprot No.：Q00604
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NDP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 102 | ||||
| Range % | 96-105 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 92-103 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 82-94 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 92-103 | |||||
回收率：
| The recovery of human NDP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 82-95 | ||||
| EDTA plasma (n=4) | 104 | 95-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.6192.5452.5822.444102.0792.0172.0481.91051.3841.3991.3921.2542.50.7330.7540.7440.6061.250.5300.5210.5260.3880.6250.2780.3010.2900.1520.3120.2070.2120.2100.07200.1390.1370.138 | ||||||
货期：3-5 working days
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